A Novel RANKL-Targeted Furoquinoline Alkaloid Ameliorates Bone Loss in Ovariectomized Osteoporosis through Inhibiting the NF-κB Signal Pathway and Reducing Reactive Oxygen Species

Figure 3

Dictamnine suppresses RANKL-induced osteoclast differentiation with no cytotoxicity in vitro. (a) The molecular formula of dictamnine. (b) Graphical representation of the source of dictamnine. (c) RAW264.7 cells seeded in a 96-well plate induced by RANKL at 50 ng/ml for 96 hours in the presence or absence of DIC. TRAP staining was performed until mature osteoclasts were produced. DIC inhibited the formation of osteoclasts in a dose-dependent manner. (d) Quantification analysis of TRAP-positive cells. (e) The cytotoxic and proliferative effects of different concentrations of DIC measured by the CCK8 method at both 24 and 48 hours. All bar charts are presented as the ; ; scale . , relative to the nontreatment group.

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