A Novel RANKL-Targeted Furoquinoline Alkaloid Ameliorates Bone Loss in Ovariectomized Osteoporosis through Inhibiting the NF-κB Signal Pathway and Reducing Reactive Oxygen Species

Figure 5

DIC attenuates RANKL-induced actin ring formation. (a) RAW264.7 cells were plated into a 96-well plate and stimulated with 20 ng/ml RANKL in the presence or absence of the indicated concentration of DIC. The cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100 solution, and stained with phalloidin (green fluorescence) and DAPI. A representative image of actin band formation was viewed with a confocal microscope, with the F-actin band stained red and the nucleus stained blue. (b) Quantitative analyses of the F-actin band and nucleus numbers per cell. All bar charts are presented as the ; ; , relative to the non-DIC treatment group.

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