Exosomal miR-224-5p from Colorectal Cancer Cells Promotes Malignant Transformation of Human Normal Colon Epithelial Cells by Promoting Cell Proliferation through Downregulation of CMTM4

Figure 4

CMTM4 is a direct binding target of miR-224-5p. (a) Schematic diagram of binding sites between miR-224-5p and CMTM4 and the mutation of binding sites in CMTM4. (b) The levels of CMTM4 in CCD 841 CoN, SW620, and HCT116 cells were assessed by RT-qPCR. vs. CCD 841 CoN group. (c) Luciferase reporter assay in SW620 cells cotransfected with WT or MT CMTM4 3UTR reporter gene and NC or miR-224-5p. (d) RT-qPCR analysis of CMTM4 level in SW620 cells transfected with NC, miR-224-5p agomir, or miR-224-5p antagomir. vs. NC group.

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