Exosomal miR-224-5p from Colorectal Cancer Cells Promotes Malignant Transformation of Human Normal Colon Epithelial Cells by Promoting Cell Proliferation through Downregulation of CMTM4

Figure 5

miR-224-5p can be transferred from SW620 cells to CCD 841 CoN cells via exosomes. (a) TEM of CCD 841 CoN cell and SW620 cell CM secreted exosomes. (b) NTA was used to determine exosome number and size distribution. (c) Western blot analysis of exosomal proteins TSG101 and CD63 in CCD 841 CoN cells, CCD 841 CoN cell-derived exosomes, and SW620 cells, SW620 cell-derived exosomes. (d) RT-qPCR analysis of miR-224-5p level in CCD 841 CoN cell-derived exosomes and SW620 cell-derived exosomes. (e) SW620 were transfected with NC, miR-224-5p agomir, or miR-224-5p antagomir for 48 h. Western blot analysis of TSG101 and CD63 levels in exosomes isolated from the transfected SW620 cells. (f) CCD 841 CoN cells were cocultured with SW620 cell-derived exosomes for 48 h. The uptake of exosomes (red color) into CCD 841 CoN cells was observed by confocal microscopy. (g) RT-qPCR analysis of miR-224-5p level in exosomes isolated from the transfected SW620 cells. vs. the CCD 841 CoN group or the Exo-NC group.

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