Exosomal miR-224-5p from Colorectal Cancer Cells Promotes Malignant Transformation of Human Normal Colon Epithelial Cells by Promoting Cell Proliferation through Downregulation of CMTM4

Figure 6

Intercellular transfer of miR-224-5p agomir by exosomes promoted malignant transformation of CCD 841 CoN cells via downregulation of CMTM4. (a) RT-qPCR analysis of CMTM4 expression in CCD 841 CoN cells transfected with CMTM4-OE. vs. NC group. (b) SW620 cells were transfected with NC, miR-224-5p agomir, or miR-224-5p antagomir for 48 h. CCD 841 CoN cells were cocultured with exosomes isolated from the transfected SW620 cells in the presence or absence of CMTM4-OE. Cell viability was determined by CCK-8 assay. (c, d) Cell proliferation detected using Ki67 immunofluorescence assay. (e, f) Transwell migration assay was applied to assess cell migration. (g, h) Transwell invasion assay was conducted to determine cell migration. (i, j) Cell apoptosis was determined using flow cytometry assay with Annexin V and PI double staining. vs. the Exo-NC group; ^## vs. the Exo-miR-224-5p agomir group.

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