Angiotensin II Promotes White Adipose Tissue Browning and Lipolysis in Mice

Figure 6

Ang II treatment enhanced AMPKα phosphorylation in adipocytes. (a, b) Western blot analyses assessing the protein expression levels of phospho-AMPKα (P-AMPKα) and AMPKα in WATs from C57BL/6 mice infused with saline or Ang II (2.5 mg/kg/d) for 2 weeks. (c) Western blot analyses assessing the protein expression levels of P-AMPKα and AMPKα in 3T3-L1 cells treated with 1 μM Ang II for the indicated time. (d) Stromal vascular fraction (SVF)/preadipocytes were isolated from SWAT of C57BL/6 mice and cultured in differentiation medium with or without Ang II treatment. Western blot analyses assessing the protein expression levels of P-AMPKα and AMPKα in these cells.

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