Hypothermia Protects against Ischemic Stroke through Peroxisome-Proliferator-Activated-Receptor Gamma

Figure 3

Hypothermia enhances the protein level of PPARγ in BV2 microglial cells and promotes microglial cells polarized from proinflammatory phenotype to anti-inflammatory phenotype through PPARγ after OGD/R. (a) Representative western blotting analysis of PPARγ in BV-2 microglial cells 24 hours after with or without 3 h-OGD for every group. Hypothermia and GW9662 (20 μM) were treated in the next 24 hours of reperfusion, respectively. GAPDH was used as a loading control. (b) Quantifications of the expression of PPARγ. (c) qRT-PCR analysis of M1-activated microglial cell markers 24 hours after with or without 3 h-OGD for every group. Hypothermia and GW9662 (20 μM) were treated in the next 24 hours of reperfusion, respectively. (d) qRT-PCR analysis of M2 activated microglial cells markers 24 hours after with or without 3 h-OGD for every group. Hypothermia and GW9662 (20 μM) were treated in the next 24 hours of reperfusion, respectively. All gene expressions were normalized to GAPDH. Experiments were repeated for 4 times. Values are presented as . ns: not significant. , , .

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