Hypothermia Protects against Ischemic Stroke through Peroxisome-Proliferator-Activated-Receptor Gamma

Figure 4

Hypothermia reduces apoptosis of neuronal cells and suppresses the neurotoxic effect of proinflammatory BV2 microglial cells on cocultured HT-22 neuronal cells through PPARγ. (a) Representative Tunnel staining of neuronal cells in the ischemic region for every group. Magnifications of the boxed areas are shown by the inset images. Scale bar: 50 μm. Magnification: 400×. (b) Quantitative analyses of the percentage of NeuN+ Tunnel+ cells in NeuN+ cells. (c) Neurological scores for every group. (d) The strategy of the cocultured system. (e) Quantifications of cell ability for every group. (f) Quantifications of LDH release ratio for every group. (g) Representative western blotting analysis of Cleaved Caspase-3, Bcl-2, and Bax for every group. GAPDH was used as the loading control. (h) Quantifications of the expression of Cleaved Caspase-3. (i) Quantifications of the expression ratio of Bcl-2/Bax. per group for (a)–(c). Experiments were repeated for 4 times for (e)–(i). Values are presented as . , , .

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