Hypothermia Protects against Ischemic Stroke through Peroxisome-Proliferator-Activated-Receptor Gamma

Figure 5

Hypothermia attenuates BBB disruption and alleviates the disruption of proinflammatory BV-2 microglial cells on cocultured bEnd.3 endothelial cells through PPARγ. (a) Representative photographs of EB extravasation for every group. (b) Quantifications of EB extravasation. (c) Representative western blotting analysis of Occludin in the ischemic region for every group. GAPDH was used as a loading control. (d) Quantifications of the expression of Occludin. (e) The strategy of the cocultured system. (f) Quantifications of cell ability for every group. (g) Quantifications of LDH release ratio for every group. (h) Representative western blotting analysis of Occludin for every group. GAPDH was used as a loading control. (i) Quantifications of the expression of Occludin. per group for (a)–(d). Experiments were repeated for 4 times for (f)–(i). Values are presented as . , , .

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