Protective Effect of Curcumin against Doxazosin- and Carvedilol-Induced Oxidative Stress in HepG2 Cells

Figure 4

Determination of necrosis marker by fluorescence microscopy of SYTOX green following doxazosin and carvedilol treatment. Doxazosin shows dose-dependent changes in the number of necrotic cells for 24 h. Carvedilol exhibit a lower number of cells dead related to the positive control for necrosis (HepG2 cells exposed to 250 μM hydrogen peroxide for 15 min). Pretreatment with curcumin maintains the viability of the cells because the staining is impermeant for apoptotic and live cells. Staining taken at 200x of magnification.