ProNGF/NGF Modulates Autophagy and Apoptosis through PI3K/Akt/mTOR and ERK Signaling Pathways following Cerebral Ischemia-Reperfusion in Rats

Figure 1

Mature NGF and proNGF’s dynamic change in the OGD/R model. Mature NGF and proNGF were detected in different reperfusion groups as follows: control cells, cells exposed to OGD without reperfusion, cells exposed to OGD/R 6 h, cells exposed to OGD/R 24 h, and cells exposed to OGD/R 48 h. Dynamic change of the NGF mRNA relative level and LDH release after OGD/R at different time points; and (a, b). Western blots of proNGF and NGF-β after OGD/R at different time points. vs control, vs OGD/R 24 h, and vs OGD/R 24 h (c). The viability of PC12 cells was measured by LDH release in seven groups as follows: control cells, cells exposed to OGD, cells exposed to OGD/R 24 h, cells exposed to OE-NGF, cells exposed to OE-NGF + OGD/R 24 h, cells exposed to si-NGF, and cells exposed to si-NGF + OGD/R 24 h. The effect of exogenous NGF-β/inhibitor and genic interference on PC12 cells after OGD/R. , ; N.S.: no significance (d, e).

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