ProNGF/NGF Modulates Autophagy and Apoptosis through PI3K/Akt/mTOR and ERK Signaling Pathways following Cerebral Ischemia-Reperfusion in Rats

Figure 2

Effect of rAdproNGF and recombinant NGF-β protein on autophagy and apoptosis of OGD/R-exposed PC12 cells. The four groups studied are as follows: control cells, cells exposed to OGD/R 24 h, cells exposed to rAdproNGF + OGD/R 24 h, and cells exposed to NGF-β + OGD/R 24 h. PC12 cells were pretransfected with GFP-LC3 plasmid and treated with rAdproNGF and recombinant NGF-β, respectively. The fluorescent staining of LC3 was observed among the groups; (a); . LC3, SQSTM1, Beclin-1, Cyto-C, PARP, ERK, Akt, and mTOR were detected by Western blots. and vs the control group; and vs OGD/R 24 h; N.S.: no significance (b). LC3, SQSTM1, Beclin-1, Cyto-C, PARP, ERK, Akt, and mTOR were detected by Western blots. vs the control group; vs OGD/R 24 h; N.S.: no significance (c). Annexin V-FITC was tested for the apoptosis; vs the OGD/R 24h group; Viability of cells was observed for rAdproNGF and recombinant NGF-β protein when exposed to autophagic inhibitor 3-MA. vs the rAdproNGF + OGD/R 24 h group. and vs the no 3-MA group; and vs OGD/R 24 h (d, e).

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