ProNGF/NGF Modulates Autophagy and Apoptosis through PI3K/Akt/mTOR and ERK Signaling Pathways following Cerebral Ischemia-Reperfusion in Rats

Figure 5

NGF regulated autophagy and apoptosis through Akt/mTOR signaling in a rat model of ischemia-reperfusion injury. Six groups were divided: sham group, PBS group, vector (null virus) group, rAdproNGF group, rWdAdproNGF group, and NGF-β group. LC3, SQSTM1, Cyto-C, PARP, ERK, Akt, mTOR, and TUNEL were detected by immunoblotting, immunohistochemistry, and immunofluorescence in control and rAdproNGF-, rWdAdproNGF-, and NGF-β-treated MCAO models. ; ; ; ; ; N.S.: no significance (a–c); . Baf-A1 was added into NGF-β therapy. Quantification of immunoblotted proteins in brain extracts was conducted for LC3, SQSTM1, Cyto-C, PARP, and mTOR. vs the tMCAO/R group; ^##P < 0.01 vs the nontreatment group; N.S.: no significance (d).

(a)

(b)

(c)

(d)