Naringin Inhibits Apoptosis Induced by Cyclic Stretch in Rat Annular Cells and Partially Attenuates Disc Degeneration by Inhibiting the ROS/NF-<i>κ</i>B Pathway

<div>Effect of naringin on oxidative stress and p-P65 expression in rat lumbar interventricular disc degeneration (IVDD) induced by unbalanced dynamic and static forces. (a–d) Effect of naringin on oxidative stress in serum and intervertebral disc tissues: (a) reactive oxygen species (ROS) production, (b) malondialdehyde (MDA), (c) superoxide dismutase (SOD), and (d) glutathione peroxidase (GPx) levels. <svg height="10.7395pt" id="M33" style="vertical-align:-0.6370001pt" version="1.1" viewbox="-0.0498162 -10.1025 50.9184 10.7395" width="50.9184pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M486 158C486 177 478 202 466 220C413 228 386 236 336 262C386 288 413 297 466 304C478 323 486 347 485 366C470 376 444 381 422 380C389 338 368 319 321 288C323 345 329 372 349 422C339 442 322 461 305 470C289 461 271 442 262 422C281 372 287 345 290 288C243 319 222 338 189 380C167 381 142 376 125 366C125 347 133 322 145 304C198 296 225 288 275 262C225 236 198 227 145 220C133 201 125 177 126 158C141 148 167 143 189 144C222 186 243 205 290 236C288 179 282 152 262 102C272 82 289 63 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transform="matrix(.013,0,0,-0.013,38.365,0)"><path d="M384 0V27C293 34 287 42 287 114V635C232 613 172 594 109 583V559L157 557C201 555 205 550 205 499V114C205 42 199 34 109 27V0H384Z"></path></g></svg> compared to the IVDD group. (e) Effect of naringin on p-P65 expression in the intervertebral discs, as observed by immunohistochemistry. The expression of p-P65 in the high- and low-concentration naringin treatment groups was lower than that in the IVDD group. The p-P65-positive cells are shown in brown (arrows). Scale bar: 1 mm (disc) and 200 <i>μ</i>m (NP and AF).</div>

Oxidative Medicine and Cellular Longevity

fig6

Figure 6

Figure 6: Naringin Inhibits Apoptosis Induced by Cyclic Stretch in Rat Annular Cells and Partially Attenuates Disc Degeneration by Inhibiting the ROS/NF-<i>κ</i>B Pathway 

Figure 6 | Naringin Inhibits Apoptosis Induced by Cyclic Stretch in Rat Annular Cells and Partially Attenuates Disc Degeneration by Inhibiting the ROS/NF-κB Pathway 