The Ethanolic Extract of Lindera aggregata Modulates Gut Microbiota Dysbiosis and Alleviates Ethanol-Induced Acute Liver Inflammation and Oxidative Stress SIRT1/Nrf2/NF-κB Pathway

Figure 2

Effect of Lindera aggregata on the expression of inflammation-related and oxidative stress-related mRNA and protein in rats with alcoholic liver injury. (a) Relative mRNA expression of SIRT1 in rat liver. (b) Relative mRNA expression of Nrf2 in rat liver. (c) Relative mRNA expression of NF-κB in rat liver. (d) Relative mRNA expression of COX2 in rat liver. (e) Western blotting was used to detect the protein levels of key molecules in the oxidative stress and inflammatory signaling pathways (SIRT1, Nrf2, NF-κB, and COX2) in the liver among the different groups. (f) SIRT1 protein level in rat liver. (g) Nrf2 protein level in rat liver. (h) NF-κB protein level in rat liver. (i) COX2 protein level in rat liver. Data are shown as the . Statistical analysis of intergroup data was performed using Tukey’s test or the Mann–Whitney test. ^#, ^##, and ^### compared with the normal group; , , and compared with the model group.

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