[Retracted] Importance of Gedunin in Antagonizing Rheumatoid Arthritis via Activating the Nrf2/ARE Signaling

Figure 2

Effect of GDN on inflammatory factors, MMPs, and ROS production in macrophages and synovial fibroblasts. (a-b) mRNA and protein levels of iNOS regulated by GDN in LPS-induced macrophages. (c-e) Inhibition of IL-1β, IL-6, and TNF-α mRNA expression by GDN in LPS-induced macrophages. (f-g) Antagonism of IL-1β, IL-6, and IL-33 mRNA levels by GDN in TNF-α-stimulated MH7A cells. (i-j) Influence of GDN on IL-1β and MMPs production. (k-l) Resistance of GDN to ROS production in RASFs. Cells were plated into 6 well plates and treated with GDN for optimized time and doses; cells total RNA and protein were collected for Q-PCR and western blotting, respectively. Living RASFs were harvested and dyed with DCFH-DA; then ROS was quantified by FACScan flow cytometer. Values of , , and were declared statistically significant vs ctrl group; , , and were declared statistically significant vs IL-1β group, LPS group, or TNF-α group.

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