Glaucocalyxin B Attenuates Ovarian Cancer Cell Growth and Cisplatin Resistance In Vitro via Activating Oxidative Stress

Figure 1

GLB increases the ROS levels and inhibits cell growth in human ovarian cancer cells. (a) The chemical formula of GLB. (b, c) A2780 or A2780/DDP cells were treated with various concentrations of GLB for 24 h. Cell viability was determined by trypan blue exclusion. (d, e) A2780 or A2780/DDP cells were exposed to GLB for 2 h; intracellular ROS levels were monitored by fluorescent probe DCFH-DA.  μm. (f) A2780 or A2780/DDP cells were treated with GLB for 20 h; the formation of 53BP1 foci was analyzed using a fluorescence microscope.  μm. Data from three technical replicates (, , and ).

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