Glaucocalyxin B Attenuates Ovarian Cancer Cell Growth and Cisplatin Resistance In Vitro via Activating Oxidative Stress

Figure 3

JNK signaling pathway is involved in GLB-induced cell death. (a, b) A2780 or A2780/DDP cells were treated with GLB at different time points. The protein levels of p-JNK were detected by western blot. (c–e) A2780 or A2780/DDP cells were treated with GLB at different concentrations. The protein levels of p-JNK were detected by western blot. (f, g) A2780 or A2780/DDP cells were pretreated with JNK inhibitor SP600125 (20 μM) for 1 h before exposing to GLB; the protein levels of p-JNK were detected by western blot. (h) A2780 or A2780/DDP cells were pretreated with JNK inhibitor SP600125 (20 μM) for 1 h before exposing to GLB; the cell viability was determined by trypan blue exclusion. (i–k) A2780 or A2780/DDP cells were pretreated with NAC for 1 h before exposing to GLB; the protein levels of p-JNK were detected by western blot. Data from three technical replicates (, , and ).

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