Glaucocalyxin B Attenuates Ovarian Cancer Cell Growth and Cisplatin Resistance In Vitro via Activating Oxidative Stress

Figure 6

NAC pretreatment reversed the combined treatment-induced DNA damage and proliferation inhibition. (a, b) A2780 or A2780/DDP cells were pretreated with NAC for 1 h and then treated with GLB or cisplatin alone or their combination for 2 h; the intracellular ROS levels were monitored by fluorescent probe DCFH-DA.  μm. (c) A2780 or A2780/DDP cells were pretreated with NAC for 1 h and then treated with GLB or cisplatin alone or their combination for 20 h; the formation of 53BP1 foci was analyzed using a fluorescence microscope.  μm. (d, e) A2780 or A2780/DDP cells were pretreated with NAC for 1 h; the colony formation ability was measured by colony-forming assay after treated with the combination. (f) A2780 or A2780/DDP cells were pretreated with NAC for 1 h and then treated with the combination for 24 h; the cell viability was determined by trypan blue exclusion. Data from three technical replicates ().

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