Glaucocalyxin B Attenuates Ovarian Cancer Cell Growth and Cisplatin Resistance In Vitro via Activating Oxidative Stress

Figure 7

JNK signaling pathway is involved in the combined treatment-induced cell death. (a, b) A2780 or A2780/DDP cells were treated with GLB and cisplatin combination at different time points. The protein levels of p-JNK were detected by western blot. (c–e) A2780 or A2780/DDP cells were treated with GLB or cisplatin alone or their combination; the protein levels of p-JNK were detected by western blot. (f, g) A2780 or A2780/DDP cells were pretreated with SP600125 for 1 h before exposing to GLB and cisplatin combination; the protein levels of p-JNK were detected by western blot. (h) A2780 or A2780/DDP cells were pretreated with SP600125 for 1 h before exposing to GLB and cisplatin combination; the cell viability was determined by trypan blue exclusion. (i–k) A2780 or A2780/DDP cells were pretreated with NAC for 1 h before exposing to GLB and cisplatin combination; the protein levels of p-JNK were detected by western blot. Data from three technical replicates (, ).

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)

(k)