Research Article
Macrophage Infiltration Reduces Neurodegeneration and Improves Stroke Recovery after Delayed Recanalization in Rats
Table 1
Details of antibodies used.
| | Antibody | Source | Dilution |
| | NeuN (rabbit) | Abcam (ab177487) | 1 : 2000 (WB), 1 : 200 (IF) | | NeuN (mouse) | Abcam (ab104224) | 1 : 2000 (WB), 1 : 200 (IF) | | Synapsin (rabbit) | Abcam (ab64581) | 1 : 1000 (WB) | | Neurofilament (rabbit) | Abcam (ab207176) | 1 : 1000 (WB), 1 : 200 (IF, IHC) | | β-Actin (mouse) | Santa Cruz (sc-47778) | 1 : 3000 (WB) | | Lectin | VectorLab (B-1175-1) | 1 : 500 (IHC), 1 : 200 (IF) | | PDGFRβ (mouse) | Abcam (ab69506) | 1 : 2000 (WB), 1 : 200 (IF) | | CD34 (rabbit) | Abcam (ab81289) | 1 : 200 (IF) | | CD45 (rabbit) | Abcam (ab40763) | 1 : 1000 (WB), 1 : 200 (IF) | | Fibronectin (rabbit) | Abcam (ab268020) | 1 : 100 (IF) | | F4/80 (rat) | Abcam (ab6640) | 1 : 1000 (WB), 1 : 200 (IF, IHC) | | PDGFB (rabbit) | Abcam (ab23914) | 1 : 1000 (WB), 1 : 200 (IF) | | VEGFA (rabbit) | Abcam (ab39250) | 1 : 1000 (WB), 1 : 200 (IF) | | HRP-goat anti-mouse | Santa Cruz (sc-2973) | 1 : 4000 (WB) | | HRP-goat anti-rabbit | Santa Cruz (sc-2357) | 1 : 4000 (WB) | | HRP-goat anti-rat | Santa Cruz (sc-362293) | 1 : 4000 (WB) | | AMCA-streptavidin | VectorLab (SA-5008-1) | 1 : 200 (IF) | | Alexa Fluor 488 goat anti-rabbit | Jackson (111-545-144) | 1 : 200 (IF) | | Alexa Fluor 568 goat anti-mouse | Jackson (115-545-166) | 1 : 200 (IF) | | Alexa Fluor 488 donkey anti-mouse | Jackson (715-545-020) | 1 : 200 (IF) | | Alexa Fluor 594 goat anti-rabbit | Jackson (111-545-003) | 1 : 200 (IF) | | Alexa Fluor 594 goat anti-rat | Jackson (112-585-003) | 1 : 200 (IF) |
|
|
WB: Western blot; IHC: immunocytochemistry; IF: immunofluorescence.
|
