Autophagy-Mediated Inflammatory Cytokine Secretion in Sporadic ALS Patient iPSC-Derived Astrocytes

Figure 2

Cytokine concentration and neurosupportive potential of astrocyte-conditioned medium (ACM) from healthy control and sporadic ALS astrocytes. (a–) ELISA-determined concentrations, pg/ml, of (a) IL1β (b) IL6, and (c) TNFα of ACM. (d) MTT-determined viability of motor neurons treated with fresh medium, normal medium, control astrocyte ACM, patient astrocyte ACM, and cytokine medium. (e) IF staining of motor neurons for the markers ChAT (choline acetyltransferase), TUJ (β-tubulin III), and DAPI. represents , represents , and represents . ELISA: enzyme-linked immunosorbent assay, MTT: 3-(4,5-dimethyl thiazolyl-2)-2,5-diphenyltetrazolium bromide.

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