Autophagy-Mediated Inflammatory Cytokine Secretion in Sporadic ALS Patient iPSC-Derived Astrocytes

Table 1

Culture medium and components.

Medium	Components
Basic neural medium	Neurobasal medium (Gibco) supplemented with 10 mM CHIR99021, 10 mM DMH-1, 10 mM SB431542, 10 mM RA, 5 mM purmorphamine, and 500 mM valproic acid (VPA)
Astrocyte induction medium	DMEM/F12 (11330-032, Thermo Fisher Scientific, MA, USA), N2 supplement (17502001, Thermo Fisher Scientific, MA, USA) and 10% fetal bovine serum (FBS) (12657029, Thermo Fisher Scientific, MA, USA)
Astrocyte maintenance medium	DMEM/F-12 and FBS (9 : 1)
PSC neural induction medium	Neurobasal medium (Gibco) supplemented with 10% neural induction supplement (Gibco)]
NSC maintenance medium	Neurobasal medium, DMEM/F-12, N2, and B27 (48.5 : 48.5 : 1 : 2)