Review Article

Unraveling the Phytochemistry, Traditional Uses, and Biological and Pharmacological Activities of Thymus algeriensis Boiss. & Reut

Table 4

In vitro activities of T. algeriensis extracts.

ExtractActivityUsed methodCountryEffectsRef

Aerial part
PE, CHCl3, and n–BuOHAntihemolyticErythrocyte osmotic fragilityAlgeria (PE), (CHCl3), (n–BuOH)[44]
Anti-inflammatoryEgg albumin denaturation (PE), 45.27 (CHCl3), 26.03 (n–BuOH)
EA & MeOHAnticorrosiveGravimetric and electrochemicalMorocco (MeOH)
[148]
EOAntitumor and cytotoxicSulforhodamine BLibya[73]
Hepatotoxicity evaluationNone of the EO showed toxicity at tested concentrations (>400 g/mL) for porcine liver primary cell culture
EOAnti-inflammatory5–LipoxygenaseAlgeria[62]
EOLeishmanicidalMTT assayTunisia[49]
Cytotoxic (murine macrophages RAW264.7)
EOACE inhibitionSpectrophotometryTunisia[29]
EOAnticorrosiveWeight loss measurementMoroccoInhibited the corrosion rate (CR) of mild steel at all concentrations ( (EO) versus 1.23 mg/cm2 h (blank) at 313 K)[137]
Potentiodynamic polarizationActed as mixed-type inhibitor
Electrochemical impedance spectroscopy
Leaves
EOPhytotoxicIn vitro seed germination inhibition (allelopathic effect)Tunisia100% inhibition of M. sativa at 1 mg/mL (dose-dependent effect)[28]
InsecticidalFumigant bioassay against Spodoptera littoralis Boisd.
EOCytotoxicMitochondrial-dependent reduction of yellowAlgeria, (cytotoxic on HCT116 cell line)[42]
(limited activity against the HePG2 cell line)
MeOHCOX inhibitionEIAAlgeria (COX–1), (COX–2)[22]
LOX inhibitionLipoxygenase inhibitor screening
MeOHCytotoxicMTTAlgeriaBiocompatible on both the immortalized tested cell lines HaCaT and BALB/c-3T3 and slightly toxic on A431 and SVT2 cancer cells at high concentrations (100 μg/mL)[47]
MeOHSilver nanoparticle biosynthesisDropwise addition of the plant extract to the silver nitrate solutionAlgeriaThe extract acts as a reducing as well as a stabilizing agent
Extract at 10% induced a consistent increase in the intensity of the surface plasmon peak absorbance for AgNPs
[71]
EtOH and EOAnticancerMTTAlgeria (EtOH), (EO)[23]
MeOHAcetylcholinesterase inhibitionSpectrophotometryTunisia[21]
Flowers & leaves
MeOH & H2OCytotoxicMTTTunisia (H2O), (MeOH)[19]
EOCytotoxicMTTTunisia (L), (F)[69]
EOAnticancerMTTMoroccoTa1 is more cytotoxic (100% lysis) than Tb2 (60% lysis) against P815 tumor cell line[93]
PBMCIncreased viability by 200%

ACE: angiotensin I-converting enzyme; CC: half maximal cytotoxic concentration; COX: cyclooxygenase; EIA: enzyme immunoassay; IC50: half maximal inhibitory concentration; IC80: concentration resulting in 80% inhibition; LC50: half maximal lethal concentration; LC90: concentration resulting in 90% lethality; LOX: lipoxygenase; MTT: methyl tetrazolium test; PBMC: peripheral blood mononuclear cells.