The Overexpression of miR-377 Aggravates Sepsis-Induced Myocardial Hypertrophy by Binding to Rcan2 and Mediating CaN Activity

Figure 5

MiR-377 increased CLP-induced cardiomyocyte hypertrophy in septic mice by inhibiting Rcan2 and regulating CaN activity through Ca²⁺-CaN pathway. (a) [Ca²⁺] in cardiomyocytes of each group. (b) The level of miR-377, Rcan2, and CaN, as measured by qRT-PCR. (c) The protein expression of Rcan2 and CaN, as examined by Western blot. (d) The result of cell proliferation in each group as examined by EdU assay. (e) The result of cell apoptosis in each group as examined by flow cytometry. (f) The expression level of inflammatory factors (TNF-α, IL-6, and IL-8) quantified by ELISA assay. compared with the mimic NC group. # compared with the miR-377 mimic group. & compared with the inhibitor NC group. @ compared with the miR-377 inhibitor group. Measurement data were showed as . Data of multiple groups were compared by one-way ANOVA with Tukey’s posthoc test. All experiments were repeated for 3 times.

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