Research Article

Upregulated IGFBP3 with Aging Is Involved in Modulating Apoptosis, Oxidative Stress, and Fibrosis: A Target of Age-Related Erectile Dysfunction

Figure 5

Verification of hub gene expression. (a, b) Immunofluorescence of IGFBP3 in the CC in age-related ED. Green represents IGFBP3. Cell nuclei were stained with DAPI (blue). (c) The mRNA expression levels of IGFBP3 were measured by qRT–PCR. (d, e) The protein expression levels of IGFBP3 were detected by western blot analysis. The data are represented as the for per group. and compared with the 16-month-old group.
(a)
(b)
(c)
(d)
(e)