Upregulated IGFBP3 with Aging Is Involved in Modulating Apoptosis, Oxidative Stress, and Fibrosis: A Target of Age-Related Erectile Dysfunction

Figure 6

Knockdown of IGFBP3 expression in CCSMCs enhanced cell viability and proliferation. (a) Morphology and immunofluorescence of α-actin in cells (magnification 400x). Green represents α-actin. (b) IGFBP3 mRNA expression was knocked down with siRNA in CCSMCs. (c, d) The protein expression levels of IGFBP3 in cells with siRNA-mediated knockdown were detected by western blot analysis. (e–g) The viability and proliferation of CCSMCs among the groups were assessed by using CCK-8 assays and EdU staining. The data are represented as the for per group. and compared with the control group.

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