AKG Attenuates Cerebral Ischemia-Reperfusion Injury through c-Fos/IL-10/Stat3 Signaling Pathway

Figure 5

The activation of IL-10/stat3 signaling was linked to the protective effect of AKG against I/R damage. (a) Western blot analysis and quantification of IL-10 and stat3 expression in the indicated groups (). (b) IL-10 levels were measured using an ELISA kit (). (c) Immunofluorescence staining () (IL-10, red; DAPI, blue). (d) Western blot analysis and quantification of IL-10 and stat3 expression in the indicated groups (). (e) IL-10 levels were measured using an ELISA kit (). (f) The levels of superoxide anions as determined by DHE staining are shown in representative photos and quantitative analysis (). (g) The levels of proinflammatory cytokines were measured using ELISA (). (a–c) , , and versus the sham group and ^#, ^##, and ^### versus the MCAO group; (d–g) and versus the control group, ^# and ^## versus the OGD/R group, and ^$ and ^$$ versus the OGD/R+AKG50 group.

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