AKG Attenuates Cerebral Ischemia-Reperfusion Injury through c-Fos/IL-10/Stat3 Signaling Pathway

Figure 6

In vitro, suppressing IL-10 greatly reduced the protective effect of AKG. (a) Western blot analysis of IL-10 expression (). (b and c) Representative pictures and quantitative analysis of superoxide anions levels as evaluated by DCFH-DA staining (). Scale bar, 100 μm. (d and e) Quantification of TUNEL-positive cells in SH-SY5Y cells in the indicated groups () (TUNEL, green; DAPI, blue). Scale bar, 100 μm. (f–h) The proinflammatory cytokines were measured using ELISA (). versus the OGD/R group and ^# versus the OGD/R+AKG50 group.

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