AKG Attenuates Cerebral Ischemia-Reperfusion Injury through c-Fos/IL-10/Stat3 Signaling Pathway

Figure 7

AKG promoted the activity and expression of c-Fos, which increased OGD/R-induced IL-10 expression. (a) An AP-1 transcription factor assay kit was used to evaluate AP-1 activity (). (b) Jund, Junb, c-Jun, Fos-b, c-Fos, Fosl2, and Fosl1 mRNA levels in different treatments by using RT-qPCR (). (c) Representative blots and quantitative analysis of IL-10 and stat3 (). (d–i) Representative blots and quantitative analysis of P-c-Fos, T-c-Fos, IL-1, IL-6, and IL-10 ( per group). (a and b) versus the OGD/R group. (e and g) versus the OGD/R group. (i) versus the OGD/R group and ^# and ^## versus the OGD/R+AKG50 group.

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