Peripheral Transplantation of Mesenchymal Stem Cells at Sepsis Convalescence Improves Cognitive Function of Sepsis Surviving Mice

Figure 3

MSCs transplantation improved hippocampal neurogenesis of sepsis survival mice. (a) Representative images of doublecortin (DCX) staining in the hippocampus on the 12th and 31th days after CLP surgery ( μm); (b) number of DCX+ cells in dentate gyrus of the hippocampus and the density of DCX+ fibers in middle molecular layer (MLm) on the 12^th and 31^th days after CLP surgery (unpaired test, sections from 3 mice per group); (c) representative western blot analysis of PSD-95 and synaptophysin (SYP) in the hippocampus on the 12^th and 31^th days after CLP surgery (One-way ANOVA followed by multiple comparison tests, mice per group). The data were expressed as ; , , . Ns: no significance; Molecular layer (ML) of DG was divided into 3 sub-regions of inner (MLi), middle (MLm), and outer (MLo). GCL indicates granule cell layer. SGZ indicates subgranular zone; Sham+NS: mice received laparotomy and tail vein injection of normal saline; Sham+MSC: mice received laparotomy and tail vein injection of MSCs; CLP + NS: mice received CLP surgery and tail vein injection of normal saline; CLP + MSC: mice received CLP surgery and tail vein injection of MSCs.

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