Peripheral Transplantation of Mesenchymal Stem Cells at Sepsis Convalescence Improves Cognitive Function of Sepsis Surviving Mice

Figure 4

MSCs transplantation alleviated microglial activation of the hippocampus of sepsis surviving mice: (a) schematic diagram of four hippocampal levels and 7 counting areas in each hippocampal level; (b) representative images of Iba-1 staining (yellow), the number of total microglial cells, and the percentage of Iba-1⁺ activated microglia in the hippocampus on the 12^th and 31^th days after CLP surgery ( μm, unpaired test, sections from 3 mice per group); (c) represent qPCR analysis of mRNA levels of inflammatory factors IL-1, IL-6, and TNF-α in the hippocampus on the 12^th day after CLP surgery (one-way ANOVA followed by multiple comparison tests, per group). The data were expressed as ; , , ; Sham+NS: mice received laparotomy and tail vein injection of normal saline; Sham+MSC: mice received laparotomy and tail vein injection of MSCs; CLP + NS: mice received CLP surgery and tail vein injection of normal saline; CLP + MSC: mice received CLP surgery and tail vein injection of MSCs.

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