Edaravone Attenuated Particulate Matter-Induced Lung Inflammation by Inhibiting ROS-NF-κB Signaling Pathway

Figure 4

EDA attenuated the PM-induced inflammatory cytokines and protein expression in lung tissues. Mice were intratracheally instilled with PM at 100 μg/day/mouse for 2 consecutive days. EDA (2, 10, or 20 mg/kg) was intraperitoneally injected prior to PM exposure for 1 h. (a) The levels of IL-6, IL-1α, and IL-1β in the BALF were measured by ELISA. (b) The level of COX-2 expression in lung tissues was measured by IHC staining. (c) The semiquantitative analysis was applied to compare the relative COX-2 protein expression. (d) COX-2 expression was determined by western blot. The optical densities of COX-2 are shown in (e). Values are the , compared with the control group; ^# or ^##, compared with the PM group; . EDA: edaravone; PM: particulate matter; BALF: bronchoalveolar lavage fluid.

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