[Retracted] Ablation of Basic Leucine Zipper Transcription Factor ATF-Like Potentiates Estradiol to Induce Atopic Dermatitis

Figure 4

17β-Estradiol induces dermatitis in male BATF^–/– mice. Male WT B6 (WT M) and BATF^–/– (BATF^–/–M) mice were injected intraperitoneally with either 1 mg/kg 17β-estradiol or sesame oil every 3 days. Estradiol treatment was started at the age of 4 weeks and ended at the age of 24 weeks. (a) A representative image of the tail skins from 2 estradiol-treated BATF^–/– mice, 1 sesame oil-treated BATF^–/– mouse, and 1 estradiol-treated WT mouse at 24 weeks of age. (b) Macroscopic scores of dermatitis in estradiol-treated BATF^–/– (), sesame oil-treated BATF^–/– (), and estradiol-treated WT () male mice at the indicated ages. Statistical significance was determined by two-way analysis of variance (ANOVA). (c) Representative H&E-stained tail skin sections from 24-week-old male BATF^–/– and WT mice, which were treated with 17β-estradiol or sesame oil as indicated. Scale bars: 400 μm (100× magnification), 200 μm (200× magnification) and 100 μm (400× magnification). (d) Total serum IgE levels detected by enzyme-linked immunosorbent assay (ELISA) from 28-week-old male BATF^–/– and WT mice with the indicated treatment ( per group). (e) Relative mRNA expressions of IL-4, IL-5, IL-1β, and IFN-γ in the tail skins of 28-week-old male estradiol-treated BATF^–/–, sesame oil-treated BATF^–/–, and estradiol-treated WT mice. , , and compared with the WT group.

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