[Retracted] Melatonin Attenuates Ischemia/Reperfusion-Induced Oxidative Stress by Activating Mitochondrial Fusion in Cardiomyocytes

Figure 2

Loss of OPA1 abolishes the cardioprotective effects of melatonin in H9C2 cells. (a) Cell viability was determined using a CCK-8 assay. (b) An ELISA was applied to analyze caspase-3 activity. (c, d) An immunofluorescence assay was used to detect troponin T levels in cardiomyocytes following H/R injury and melatonin treatment. (e)–(g) ELISAs were used to measure cardiac damage markers such as LDH, troponin T, and CK-MB. vs. control.

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