α-Ketoglutarate Improves Meiotic Maturation of Porcine Oocytes and Promotes the Development of PA Embryos, Potentially by Reducing Oxidative Stress through the Nrf2 Pathway

Figure 2

Effects of α-KG on the formation of ROS and GSH in mature oocytes. (a) Representative pictures of reactive oxygen species and glutathione staining. (b) Analysis of relative fluorescence intensity of ROS in the control group and the α-KG-supplemented group. (c) Analysis of relative fluorescence intensity of GSH in the control group and the α-KG-supplemented group. (A, B) , , and ; SEM: standard error of mean. (d) The effect of adding α-KG on the mRNA expression of antioxidant-related genes in mature oocytes. ; SEM: standard error of mean. (e) Relative mRNA expression of oocyte maturation-related genes (BMP15, PFKP, GPX1, GSS, Gas6, and Gdf9) in the control group and the α-KG addition group; ; SEM: standard error of mean.

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