Epigenome-Wide Association Study Reveals Differential Methylation Sites and Association of Gene Expression Regulation with Ischemic Moyamoya Disease in Adults

Figure 3

(a) Expression of KCNMA1, GALNT2, SOX6, and RBM33 in HBMECs were determined using RT-qPCR. The results are presented as (). . (b) EdU assay of HBMECs. The proliferating nuclei were stained red with EdU and blue with Hoechst for 2 h. Three random pictures per group were used to count the numbers of positive cells and Hoechst-positive cells. Data are shown as the from three independent experiments (). , . (c) Growth of HBMECs after the overexpression of KCNMA1 or GALNT2 and knocking down of SOX6 or RBM33. Cell viability was measured using the CCK8 assay after 24 h, 48 h, and 72 h incubation. The results were presented as (). , . (d) Apoptosis in HBMECs was assessed using flow cytometry after staining with AnnexinV and PI (a). Data are shown as the from three independent experiments. (e) Capillary formation was measured using the tube formation assay. HBMECs from each group were seeded on Matrigel-coated wells and incubated for 72 h to allow the formation of capillary-like structures (). The quantitative data of tube formation assay (b, c). .

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