Epoxyeicosatrienoic Acids Inhibit the Activation of Murine Fibroblasts by Blocking the TGF-β1-Smad2/3 Signaling in a PPARγ-Dependent Manner

Figure 3

The CYP2J2 overexpression attenuates TGF-β1-induced activation of murine fibroblasts. The expression of CYP2J2 mRNA in NIH3T3 cells after the adenovirus-CYP2J2 infection for 48 h was detected by PCR ((a, b), ). The cell proliferation was examined by CCK-8 ((c), ). Western blot was applied to detect the protein level of α-SMA and PCNA in NIH3T3 cells from each group ((d)–(f)(d)–(f), ). , , and .

(a)

(b)

(c)

(d)

(e)

(f)