Epoxyeicosatrienoic Acids Inhibit the Activation of Murine Fibroblasts by Blocking the TGF-β1-Smad2/3 Signaling in a PPARγ-Dependent Manner

Figure 4

EETs attenuate TGF-β1-induced activation of murine fibroblasts. NIH3T3 cells were treated with 14,15-EET (1 μM) for 5 min, followed by treatment with TGF-β1 (10 ng/mL) for 48 h. The cell proliferation was examined by CCK-8 ((a)–(c), ). Western blot was applied to detect the protein level of COL-III, α-SMA, and PCNA in NIH3T3 cells from each group ((d)–(g), ). Cell cycles were detected using flow cytometry ((h)–(j), ). , , and . ### vs. TGF-β1 group.

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