Epoxyeicosatrienoic Acids Inhibit the Activation of Murine Fibroblasts by Blocking the TGF-β1-Smad2/3 Signaling in a PPARγ-Dependent Manner

Figure 6

PPARγ inhibitor abolishes the effects of 14,15-EET on the activation of murine fibroblasts. NIH3T3 cells were treated with the GW9662 (10 μM) for 1 h, followed by treatment with 14,15-EET (1 μM) for 5 min and TGF-β1 (10 ng/mL) for 48 h. α-SMA and PCNA in NIH3T3 cells were detected by western blot ((a)–(c), ). Cells were treated with the GW9662 (10 μM) for 1 h, followed by treatment with 14,15-EET (1 μM) for 5 min and TGF-β1 (10 ng/mL) for 30 min. p-Smad2 and p-Smad3 were detected by western blot ((d)–(f), ). , , and .

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