Hydrogen Sulfide Suppresses Skin Fibroblast Proliferation via Oxidative Stress Alleviation and Necroptosis Inhibition

Figure 10

NaHS suppressed α-SMA expression in TGF-β₁-stimulated fibroblasts. Skin fibroblasts were extracted from skin tissues of normal controls (normal skin). After pretreatment with NaHS (50 μM) for 4 h, skin fibroblasts were stimulated with TGF-β₁ (10 ng/mL) for 12 h. (a) α-SMA mRNA expression was detected with real-time PCR. (b) α-SMA was immunofluorescence stained with Alexa Fluor 488- (green) conjugated IgG. The nuclei were stained with DAPI (blue). . (c) Quantitative statistics of α-SMA fluorescence intensity. (d) α-SMA protein expression was detected by Western blot. vs. normal skin; vs. TGF-β₁, .

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