Hydrogen Sulfide Suppresses Skin Fibroblast Proliferation via Oxidative Stress Alleviation and Necroptosis Inhibition

Figure 11

NaHS inhibited PCNA expression in TGF-β₁-stimulated fibroblasts. Skin fibroblasts were extracted from skin tissues of normal controls (normal skin). After pretreatment with NaHS (50 μM) for 4 h, skin fibroblasts were stimulated with TGF-β₁ (10 ng/mL) for 12 h. (a) PCNA was immunofluorescence stained with Alexa Fluor 488- (green) conjugated IgG. The nuclei were stained with DAPI (blue). . (b) Quantitative statistics of PCNA fluorescence intensity. (c) PCNA protein expression was detected by Western blot. vs. normal skin; vs. TGF-β₁, .

(a)

(b)

(c)