Hydrogen Sulfide Suppresses Skin Fibroblast Proliferation via Oxidative Stress Alleviation and Necroptosis Inhibition

Figure 12

NaHS prevented collagen synthesis in TGF-β₁-stimulated fibroblasts. Skin fibroblasts were extracted from skin tissues of normal controls (normal skin). After pretreatment with NaHS (50 μM) for 4 h, skin fibroblasts were stimulated with TGF-β₁ (10 ng/mL) for 12 h. (a) Collagen I mRNA expression was detected with real-time PCR. (b) Collagen I was immunofluorescence stained with Alexa Fluor 488- (green) conjugated IgG. The nuclei were stained with DAPI (blue). . (c) Collagen III mRNA expression was detected with real-time PCR. (d) Collagen III was immunofluorescence stained with Alexa Fluor 488- (green) conjugated IgG. The nuclei were stained with DAPI (blue). . vs. normal skin; vs. TGF-β₁, .

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