Hydrogen Sulfide Suppresses Skin Fibroblast Proliferation via Oxidative Stress Alleviation and Necroptosis Inhibition

Figure 14

NaHS alleviated necroptosis in TGF-β₁-stimulated skin fibroblasts. Skin fibroblasts were extracted from skin tissues of normal controls (normal skin). After pretreatment with NaHS (50 μM) for 4 h, skin fibroblasts were stimulated with TGF-β₁ (10 ng/mL) for 12 h. (a) Necroptotic cells were marked with TUNEL staining (green). The nuclei were stained with DAPI (blue). . (b) Quantitative statistics of TUNEL-positive cells. (c–e) Expression of RIPK1, RIPK3, and MLKL was detected by Western blot. vs. normal skin; vs. TGF-β₁, .

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