Hydrogen Sulfide Suppresses Skin Fibroblast Proliferation via Oxidative Stress Alleviation and Necroptosis Inhibition

Figure 4

NaHS inhibited PCNA expression in keloid fibroblasts. Skin fibroblasts were extracted from skin tissues of normal controls (normal skin) and scar tissue from patients with keloid (keloid), respectively. After pretreatment with NaHS (50 μM) or the same amount of culture medium (control) for 4 h, the fresh culture medium was replaced. (a) After 12 h, PCNA was immunofluorescence stained with Cy3- (red) conjugated IgG. The nuclei were stained with DAPI (blue). . (b) Quantitative statistics of PCNA fluorescence intensity. (c) Expression of PCNA protein was detected by Western Blot. vs. normal skin + control; vs. keloid + control, .

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