Hydrogen Sulfide Suppresses Skin Fibroblast Proliferation via Oxidative Stress Alleviation and Necroptosis Inhibition

Figure 5

NaHS blocked collagen synthesis in keloid fibroblasts. Skin fibroblasts were extracted from skin tissues of normal controls (normal skin) and scar tissue from patients with keloid (keloid), respectively. After pretreatment with NaHS (50 μM) or the same amount of culture medium (control) for 4 h, the fresh culture medium was replaced. (a) After 12 h, collagen I mRNA expression was detected with real-time PCR. (b) Collagen I was immunofluorescence stained with Cy3- (red) conjugated IgG. The nuclei were stained with DAPI (blue). . (c) Collagen III mRNA expression was detected with real-time PCR. (d) Collagen III was immunofluorescence stained with Cy3- (red) conjugated IgG. The nuclei were stained with DAPI (blue). . vs. normal skin + control; vs. keloid + control, .

(a)

(b)

(c)

(d)