Hydrogen Sulfide Suppresses Skin Fibroblast Proliferation via Oxidative Stress Alleviation and Necroptosis Inhibition

Figure 6

NaHS attenuated oxidative stress in keloid fibroblasts. Skin fibroblasts were extracted from skin tissues of normal controls (normal skin) and scar tissue from patients with keloid (keloid), respectively. After pretreatment with NaHS (50 μM) or the same amount of culture medium (control) for 4 h, the fresh culture medium was replaced. (a) After 12 h, intracellular superoxide anion was stained by DHE (red). The nuclei were stained with DAPI (blue). . (b) Mitochondrial superoxide was stained with MitoSOX (red). The mitochondria were stained with MitoTracker (green). The nuclei were stained with DAPI (blue). .

(a)

(b)