Hydrogen Sulfide Suppresses Skin Fibroblast Proliferation via Oxidative Stress Alleviation and Necroptosis Inhibition

Figure 7

NaHS improved mitochondrial membrane potential in keloid fibroblasts. Skin fibroblasts were extracted from skin tissues of normal controls (normal skin) and scar tissue from patients with keloid (keloid), respectively. After pretreatment with NaHS (50 μM) or the same amount of culture medium (control) for 4 h, the fresh culture medium was replaced. After 12 h, mitochondrial membrane potential was detected by JC-1 staining. JC-1 aggregates (red) and JC-1 monomers (green) were observed. The nuclei were stained with DAPI (blue). .