Hydrogen Sulfide Suppresses Skin Fibroblast Proliferation via Oxidative Stress Alleviation and Necroptosis Inhibition

Figure 8

NaHS alleviated necroptosis in keloid fibroblasts. Skin fibroblasts were extracted from skin tissues of normal controls (normal skin) and scar tissue from patients with keloid (keloid), respectively. After pretreatment with NaHS (50 μM) or the same amount of culture medium (control) for 4 h, the fresh culture medium was replaced. (a) After 12 h, necroptotic cells were marked with TUNEL staining (green). The nuclei were stained with DAPI (blue). . (b) Quantitative statistics of TUNEL-positive cells. (c–e) Expression of RIPK1, RIPK3, and MLKL was detected by Western blot. vs. normal skin + control; vs. keloid + control, .

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