Actin Alpha 2 Downregulation Inhibits Neural Stem Cell Proliferation and Differentiation into Neurons through Canonical Wnt/β-Catenin Signaling Pathway

Figure 1

Primary NSC culture and characteristics. (a) The suspended neurospheres were notably floating in enrichment medium on day 3. Scale bar: 100 μm. (b) Immunostaining images indicating most of cultured cells expressed Nestin and Sox2. Cell nuclei were counterstained with DAPI in blue. Scale bar: 20 μm. (c) Immunostaining images showing cultured cells bore the multipotency of differentiation into MAP2⁺ cells. Cell nuclei were counterstained with DAPI in blue. Scale bars: 10 μm. (d) Immunostaining images demonstrating cultured cells held the ability of differentiation into GFAP⁺ cells. Cell nuclei were counterstained with DAPI in blue. Scale bars: 10 μm. (e) Immunostaining images delineating cultured cells possessed the capacity of differentiation into Olig2⁺ cells. Cell nuclei were counterstained with DAPI in blue. Scale bar: 10 μm.

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